Colorimetric method for Potassium

First of all let me define first what does a colorimeter do.

According to wikipedia, A Colorimeter is a device used in colorimetry. In scientific fields the word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. This device is most commonly used to determine the concentration of a known solute in a given solution by the application of the Beer-Lambert law, which states that the concentration of a solute is proportional to the absorbance.

The assigned topic to me is colorimetric method for Potassium determination, before I start explaining the method I will explain first what is Potassium. Potassium is an element (and an electrolyte) that's essential for the body's growth and maintenance. It's necessary to keep a normal water balance between the cells and body fluids. Potassium also plays an essential role in the response of nerves to stimulation and in the contraction of muscles. Cellular enzymes need potassium to work properly.

There are many methods or test wherein you can use the colorimeter and one of these test is the Potassium Determination. This colorimetric method for the direct determination of potassium in serum or plasma is based on the selective complexing of potassium by a specific macrocyclic polyether, with the subsequent formation of an ion-pair with a colored anion. The colored anion is extracted into an organic solvent, clarified by centrifugation, and then measured at 415 nm. The absorbance of the chromogen varies linearly with [K+] to at least 15 mmol/L. Results of this colorimetric method (y) correlate well with the results obtained by a flame- photometric method (y = 1.04x - 0.22, r = 0.97, n = 81), with CVs ranging from 2 to 4%. We observed no interferences from lipemia, added bilirubin, or various electrolytes. We also evaluated the use of this reagent in a new automated blood analyzer developed by Abbott, a two- dimensional centrifugal system (Clin Chem 31:1457-1463, 1985). Potassium determined with this system (y) correlated well with results by flame photometry: y = 1.02x + 0.02 (r = 0.94, n = 168). With this system one can use whole-blood specimens in measuring potassium. 

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References: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC437895/

http://en.wikipedia.org/wiki/Colorimeter

http://www.wisegeek.com/what-is-a-colorimeter.htm

Hard work

My definition of hard word is that which challenges you.

Most of the people choose the easiest instead of choosing the hard one. Why?

Because they think that it will benefit them that much. That's why you readers should do the opposite. The opportunities of life will be attacked of bunch of people who are seeking what's easy but they don't know that the much tougher challenges will usually see a lot less competition and a lot more opportunity.

Thinking back of my high school years, I spent all my time studying my lessons because at that time, I believed that if you want to become someone on the future you should work hard to earn what you really want. My point here is that tough/strong challenge is connected with strong results. You can get lucky every once in a while and find an easy path to success.

But the question is..

Will you able to maintain that success or it's just luck that made you there?

Will you able to repeat it?

The willingness to do what is difficult is like having a key to a special private treasure room. Just like the image above says, All that hard work PAID OFF. It is really true that hard work pays off. The greater our capacity to hard work is, the more rewards will fall within our grasp. The dipper we dig the more treasure we will find.

So, as to end this article I strongly advice you guys that stop avoiding and fearing hard work and simply surrender it. Make it your ally instead of your enemy. It's a potential to have it on your side. 

"The big secret in life is that there is no big secret. Whatever your goal, you can get there if you’re willing to work."

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Hi..Hello..

Hello world it's me Jeh.. Let's begin the

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“Learn from yesterday, live for today, hope for tomorrow.”

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